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Expression of Nampt, RelB, Bax, PARP-1 was detected by western blot and hippocampal primary neuronal apoptosis by Flow cytometry. A. P7C3 attenuated Sev-N2O anesthesia induced hippocampal primary neuronal apoptosis. B. Nampt and RelB protein were up-regulated, and Bax, PARP-1 proteins were down-regulated by P7C3 stimulation in advance compared with Sev-N2O group.
