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. 2018 Jun 1;11(6):2980–2989.

Figure 4.

Figure 4

miR-27a targets NF-κB signaling pathway member TAB3. A. The target regions of the miR-27a in TAB3 was predicted using Targetscan. B. A luciferase reporter plasmid containing wild TAB3 3’UTR or mutant TAB3 3’UTR was transfected into 293T cells alone, together with miR-27a mimic, inhibitor or controls and luciferase activity was measured. C. qRT-PCR detection of TAB3 mRNA expression after administration of miR-27a mimics or miR-27a inhibitor. D. Western blot detection of TAB3 protein expression after administration of miR-27a mimics or miR-27a inhibitor in. Data are shown as mean ± SD (n = 3). **P<0.01 vs. mimics control group; ##P<0.01 vs. inhibitor control group.