Figure 6.

Overexpression of miR-182-5p blocked TLR4/NF-κB pathway in SCI cell model. C8-D1A cells were co-transfected with miR-182-5p mimics and pcDNA-TLR4 vector for 48 hours and then exposed to 200 μM H₂O₂ for 10 h. A. The levels of TLR4, nuclear p-p65, and p-IκB-α were measured by Western blot. B. The bands were semi-quantitatively analyzed by using Image J software, normalized to β-actin density. Data represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 vs. H₂O₂ alone group. ##P < 0.01 vs. H₂O₂ + miR-182-5p mimics group.