Figure 4.

miR-142-3p inhibited HG-induced EndMT by targeting TGF-β1. pcDNA-TGF-β1 and miR-142-3p mimics were co-transfected into HAECs, followed by HG treatment for 5 days, after which the cells were harvested for subsequent experiment. A-D. The mRNA expression levels of α-SMA, vimentin, CD31 and VE-cadherin were measured by qRT-PCR. E. The protein expression levels of α-SMA, vimentin, CD31 and VE-cadherin were measured by Western Blot. Data represent the mean ± SD of three independent experiments. ##P < 0.01 vs. HG + mimics group.