Figure 4.

The β3 subunit controls the level of BSP and the expression of RUNX2 and collagen I as determined by RT-PCR, WB and immunofluorescence staining, respectively. A and C. The mRNA expression of RUNX2 and collagen I were determined by RT-PCR. B and D. The protein expression of RUNX2 and collagen I were determined by WB. E. The expression of the osteogenic marker, bone sialoprotein (BSP), was investigated with immunofluorescence staining. The cells were treated with a β3 antibody or a β3 plasmid for 21 days and then stained with DAPI.