Table 1.
Detailed list of the primers used in this study.
| Primer Designation | Primers Sequence | Target Gene | Location * | Size Product | Reference |
|---|---|---|---|---|---|
| MdCv-F MdCv-R |
5′-GTGATGGGAAGGCGATAGAA-3′ 5′-TCCGCATATGTTCCTCCTTC-3′ |
pp38 | 127525–127506 A 127300–127319 A |
226 bp | [8] |
| SNV-LTR-F SNV-LTR-R |
5′-AATGGTTGTAAAGGGCAGAT-3’ 5’-CTCCTCTCACTGCCAATCT-3′ |
LTR (REV) | 267-286/8012–8031 B 466–448/8211–8193 B |
201 bp | [8] |
| Leu3.2F Leu7R |
5′-GGAAATGTAGTGTTATRCRATACTCTTATG-3′ 5′-ATCCGCTTCATGCAGGTGCTC-3′ |
LTR (ALV) | 7514–7543 C 7813–7834 C |
321 bp | [18] |
| Leu11F Leu12R |
5′-CGTCGATTGGTGGAAGTAAGGTGG-3′ 5′-TCA GGG AAT CGA CGG TCC GGC C-3′ |
LTR (ALV) | 7594–7617 C 7785–7806 C |
213 bp | [18] |
| CVI988-F Non-CVI988-F MDV pp38-R |
5′-GAGGGAGAGTGGCTGTCAAG-3′ 5′-GAGGGAGAGTGGCTGTCAAA-3′ 5′-TCCGCATATGTTCCTCCTTC-3′ |
pp38 | 127487–127468 A 127487–127468 A 127300–127319 A |
188 bp | [19] |
| MDVgB-gF1 MDVgB-gR1 |
5′-CCCATRCCGTTRAACAATTC-3′ 5′-GTYCAATTCGCCATGCTCCA-3′ |
gB | 61554–61573 A 62281–62262 A |
728 bp | This study |
| REV-Pol1-F5 REV-Pol1-R5 |
5′-ACTCGCCCAGGAGAGTAGAG-3′ 5′-GAATAGTTTCGCGCAGGCTT-3′ |
gag + pol | 2269–2288 B 3035–3016 B |
767 bp | This study |
| REV-Env3-F12 REV-Env3-R12 |
5′-GTGCATACTGGCATCAATCG-3′ 5′-CCACATTCCCCACYGCTCTT-3′ |
env | 7050–7069 B 7752–7733 B |
703 bp | This study |