Figure 3.

Triacsin C blocks the rescue of Malassezia FAA1 in S. cerevisiae faa1 and faa4 mutant. A CM ura⁻ plate containing cerulenin (5 μg/mL), C14:0 + C16:0 (each 0.5 mM), and triacsin C (3 μM) was prepared. An agar plate containing C14:0 + C16:0 and triacsin C without cerulenin, and an agar plate containing cerulenin and triacsin C were used as positive and negative controls, respectively. S. cerevisiae strains were grown at 30 °C for 3–5 days.