Figure 5.

The expression of the small heat-shock proteins hspb9 and hspb11 are increased in propoxur-treated embryos. (A) Heat map of 56 genes that were identified by microarray analysis to be significantly regulated by propoxur treatment: Each column represents a separate biological pool of 40 hpf embryos treated with either DMSO or propoxur. Dark blue represents a 2-fold downregulation of expression, whereas dark red represents a 2-fold upregulation in expression. Expressions of hspb9 and hspb11 are indicated by red arrows. (B,C) qRT-PCR confirmation of hspb9 and hspb11 upregulation following propoxur treatment and heat shock: The asterisks represent different levels of significance (p values are shown).