Figure 13.

Western blot analysis showing the effects of IC₅₀ doses of DoHuRu/POPC and DoHuRu/DOTAP following 48 and 72 h of incubation in MCF-7 (a) and MDA-MB-231 (b) cells on caspases-3, -8 and -9 expression and activation, and on Bax and BCL-2 expression. Bands resulting after chemoluminescence from treated cells were extracted, quantified by densitometric analysis and then plotted (graphs on the right of each panel): solid and dotted lines for Bax and BCL-2 expression proteins, respectively, and bar graphs (caspases-3, -8 and -9) reported as percentage of control in relation to the used Ru-containing nanoaggregates. Figures are adapted from [134].