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. 2020 Jan 9;180(1):92–106.e11. doi: 10.1016/j.cell.2019.11.032

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© 2019 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S2 — Production of Recombinant HAs, Related to Figure 2

Cysteine-stabilized HAs (Lee et al., 2015) were produced in CHO cells and purified as detailed in the STAR Methods. Left, stained SDS-PAGE gel of H1_PR8 under reducing and non-reducing conditions. HA is shown prior to and after thrombin digestion to remove trimerization, biotinylation, AviTag, and HisTag domains. Right, non-reducing SDS-PAGE gel of HAs used for heterologous boosting (pandemic H1 A/California/07/2009 (H1_Cal), and H5 A/Indonesia/05/2005 (H5_Ind)) after thrombin digestion. Both strips are cropped from the same gel.