Figure S4.
Clonal Analysis of the Response to HA Immunization Following Influenza Infection, Related to Figure 5
(A) Clonal maps showing distribution of clones across different compartments 6 days post-boost. Data as in Figure 5C, showing two additional mice. In mouse 4, FM+ cells were present in only one pLN GC.
(B) Quantification of clonal dominance (N75) and total diversity (Chao1) in different compartments for all 4 mice. Data are for two AID-Confetti and two S1pr2-Tomato mice (Figures 5C and S4A). Each symbol represents one mouse, except for S1pr2-Tomato pLN cells, where both FPs were boosted and each symbol represents one pLN. One sample of FM+ pLN GC B cells from S1pr2-Tomato was omitted from the analysis due to low cell count. Bar represents median.
(C) Average VH mutations per clone in fate-mapped and non-fate-mapped GC B cells from Figures 5C and S4A (all four mice are pooled). P value is for Mann-Whittney U test.
(D) Proportion of cells (left) and clones (right) found in the “used” MBC fraction (blue and green colors in Figures 5C and S4A). P value is for one-way ANOVA with Dunnett’s multiple comparisons test.