Figure S5.

Comparative Clonal Composition of the Secondary Response to HA, Related to Figure 5

(A) Clonal sharing between footpads in fate-mapped and non-fate-mapped secondary GC B cells. S1pr2-Tomato mice were infected with influenza then boosted in both hind footpads with recombinant HA as described in Figure 5. Figures show the clonal composition of non-fate-mapped GC B cells (left panel) and fate-mapped MBC, PB, and GC B cells (right panel) in the two pLNs at 6 days after boost. Clones found in both left and right pLN are highlighted in blue. Data correspond to mice 3 and 4 from Figures 5A and S4A.

(B) Clonal maps showing distribution of clones across different compartments. Data as in Figure 5C but analyzed at day 9 post boost. ^∗MBCs and PBs for mouse 6 were not analyzed due to the very low number of cells.

(C) Quantification of clonal diversity and dominance for the data in (B).

(D) Average V_H mutations per clone in fate-mapped and non-fate-mapped GC B cells from (B). P value is for Mann-Whittney U test.