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. 2020 Jan 13;20:18. doi: 10.1186/s12935-020-1100-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Microscopy and Flow cytometry analysis of MDA-MB-231 cells after pre-irradiation and treatment with GA for 24 h. panel I: invert microscopy images (×40), panel II: AO/EB double staining, panel III: the apoptotic rates (annexin V-FITC/PI dual staining) and panel IV: colony-forming ability. a 0 µg/ml (control), and b 25 µg/ml of GA at dark condition, c 0 µg/ml and d 25 µg/ml of GA under low-level laser irradiation. e Histogram showing percentages of apoptotic and necrotic cells in the dark and irradiation groups treated with irradiation and GA. Percent of apoptotic and necrotic cells obtained from (panel III)