Fig. 2.

Gli inhibition reduces EMT activity in NSCLC cell lines. a MTS cell viability assay in A549 and H1975 NSCLC cell lines. Cells were subjected to a serial dilution of Gli-i with DMSO control over a 3-day period, yielding IC₅₀ values of 9.385 nM and 13.61 nM, respectively. b Luciferase reporter assay in H1975 cell lines, treated with DMSO (control), N-Shh (0.5 mg/mL), GANT61(15umol/L), or GANT61(15umol/L) and N-Shh (0.5 mg/mL) stimulation, for 24 h. Results are expressed as fold activity, i.e. the ratio of luciferase activity induced in Gli-transfected cells relative to basal luciferase activity in control transfected H1975 cells (p-values of < 0.05 was indicated as *)