Fig. 3.

Circ-ABCB10 regulates AK4 expression by sponging miR-556-3p in lung cancer. a Venn pattern showed the overlap of the prediction results of four databases. b-c AK4 expression in transfected cells were examined through RT-qPCR together with western blot. d The expression of AK4 in lung cells and HBE cells was observed via RT-qPCR and western blot. e Measurement of RNA enrichments through RIP and RT-qPCR. f A binding site between miR-556-3p and AK4 predicted by starBase was shown. g The efficiency of AK4 overexpression was analyzed via RT-qPCR. h The interaction between RNAs was confirmed by luciferase reporter assay. i The efficiency of AK4 knockdown was analyzed via RT-qPCR. j-m Cell proliferation, apoptosis and migration in different groups were separately evaluated by CCK-8, colony formation, TENEL and transwell assays. ^*P < 0.05, ^**P < 0.01