Table 1.
Experimental design (conditions and responses) for BPA degradation by Pseudomonas putida YC-AE1
| Run No. | A | B | C | D | E | BPA degradation (%)a |
|---|---|---|---|---|---|---|
| Initial pH (values) | NaCl Conc. (%) | Incubation period (hours) | Inoculum size (%) | Incubation temp. (°C) | ||
| 7 | 5.00 (−2) | 0.75 (0) | 20 (0) | 2.5 (0) | 31 (0) | 2.2 |
| 5 | 6.13 (−1) | 1.13 (+ 1) | 15 (− 1) | 3.25 (+ 1) | 37 (+ 1) | 2.7 |
| 8 | 6.13 (−1) | 1.13 (+ 1) | 15 (−1) | 1.75 (− 1) | 25 (− 1) | 7.8 |
| 10 | 6.13 (−1) | 0.38 (− 1) | 25 (+ 1) | 1.75 (− 1) | 25 (− 1) | 81.7 |
| 11 | 6.13(− 1) | 1.13 (+ 1) | 25 (+ 1) | 1.75 (− 1) | 37 (+ 1) | 0 |
| 12 | 6.13 (−1) | 0.38 (− 1) | 15 (− 1) | 1.75 (− 1) | 37 (+ 1) | 0 |
| 14 | 6.13 (− 1) | 1.13 (+ 1) | 25 (+ 1) | 3.25 (+ 1) | 25 (− 1) | 6.4 |
| 28 | 6.13 (−1) | 0.38 (− 1) | 25 (+ 1) | 3.25 (+ 1) | 37 (+ 1) | 13.38 |
| 29 | 6.13 (−1) | 0.38 (− 1) | 15 (− 1) | 3.25 (+ 1) | 25 (− 1) | 93.5 |
| 1 | 7.25 (0) | 0.75 (0) | 10 (−2) | 2.5 (0) | 31 (0) | 2 |
| 2 | 7.25 (0) | 1.5 (+ 2) | 20 (0) | 2.5 (0) | 31 (0) | 39.4 |
| 3 | 7.25 (0) | 0.75 (0) | 20 (0) | 2.5 (0) | 31 (0) | 77 |
| 4 | 7.25 (0) | 0.75 (0) | 20 (0) | 2.5 (0) | 19 (−2) | 17.8 |
| 15 | 7.25 (0) | 0.75 (0) | 20 (0) | 2.5 (0) | 31 (0) | 77.2 |
| 16 | 7.25 (0) | 0.75 (0) | 30 (+ 2) | 2.5 (0) | 31 (0) | 90 |
| 19 | 7.25 (0) | 0.75 (0) | 20 (0) | 4 (+ 2) | 31 (0) | 63.2 |
| 20 | 7.25 (0) | 0 (−2) | 20 (0) | 2.5 (0) | 31 (0) | 90 |
| 22 | 7.25 (0) | 0.75 (0) | 20 (0) | 2.5 (0) | 31 (0) | 77.3 |
| 23 | 7.25 (0) | 0.75 (0) | 20 (0) | 2.5 (0) | 43 (+ 2) | 0 |
| 24 | 7.25 (0) | 0.75 (0) | 20 (0) | 1 (−2) | 31 (0) | 17.5 |
| 6 | 8.38 (+ 1) | 0.38 (−1) | 25 (+ 1) | 1.75 (−1) | 37 (+ 1) | 1.7 |
| 9 | 8.38 (+ 1) | 1.13 (+ 1) | 15 (−1) | 3.25 (+ 1) | 25 (− 1) | 17.5 |
| 13 | 8.38(+ 1) | 1.13 (+ 1) | 25 (+ 1) | 1.75 (−1) | 25 (− 1) | 15 |
| 18 | 8.38(+ 1) | 0.38 (−1) | 15 (−1) | 3.25 (+ 1) | 37 (+ 1) | 7 |
| 21 | 8.38 (+ 1) | 0.38 (−1) | 25 (+ 1) | 3.25 (+ 1) | 25 (− 1) | 95 |
| 25 | 8.38 (+ 1) | 1.13 (+ 1) | 15 (−1) | 1.75 (− 1) | 37 (+ 1) | 0 |
| 26 | 8.38 (+ 1) | 1.13 (+ 1) | 25 (+ 1) | 3.25 (+ 1) | 37 (+ 1) | 0 |
| 27 | 8.38 (+ 1) | 0.38 (−1) | 15 (− 1) | 1.75 (− 1) | 25 (− 1) | 78.3 |
| 17 | 9.5 (+ 2) | 0.75 (0) | 20 (0) | 2.5 (0) | 31 (0) | 60 |
aAll response (BPA degradation percentage) values are shown as means for three independent experiments