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. 2020 Jan 13;20:20. doi: 10.1186/s12935-020-1101-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effect of EphA5 knockdown on EMT was mediated by activating Wnt/β‑catenin signaling pathway. a, b Western blot was used to determine the expression of the key factors in Wnt/β‑catenin signaling (total and phosphorylated GSK-3β, β-catenin), and the downstream targets of Wnt/β‑catenin signaling(c-Myc and CyclinD1) in KYS150 and KYSE450 cells. c, d Immunofluorescence analysis showed β-catenin and phosphorylated GSK-3β localization and expression. Nuclei were counterstained with DAPI (blue). *P < 0.05, **P < 0.01, ***P < 0.001 versus NC group