Table 3.

qRT-PCR of specific pilin-associated genes to validate RNA-Seq analyses

	RNA-Seq fold changes			qRT-PCR ΔΔCt fold changes
	BHI PL/LI	PGY PL/LI	FABG PL/LI	BHI PL/LI	PGY PL/LI	FABG PL/LI
pilA2 (cpe2284)	0.873*	1.326	2.339	1.21–1.69	1.07–2.05	1.33–2.93
pilB2 (cpe2286)	1.143	2.950	5.233	0.74–1.44	1.07–3.62	2.98–5.68
pilC2 (cpe2285)	0.807	3.063	4.178*	0.79–2.29	1.95–3.41	1.52–3.02
pilT (cpe1767)	0.614*	1.735	0.942	0.77–1.28	1.27–2.09	0.87–1.64
cpe2277	0.679*	3.128	1.699*	0.72–1.87	1.82–3.18	0.88–1.61
lon (cpe2635)	0.795	0.939	1.130

*indicate a value out of fold change range but shows similar trends