Skip to main content
. 2020 Jan 14;21:45. doi: 10.1186/s12864-020-6453-z

Table 3.

qRT-PCR of specific pilin-associated genes to validate RNA-Seq analyses

RNA-Seq fold changes qRT-PCR ΔΔCt fold changes
BHI PL/LI PGY PL/LI FABG PL/LI BHI PL/LI PGY PL/LI FABG PL/LI
pilA2 (cpe2284) 0.873* 1.326 2.339 1.21–1.69 1.07–2.05 1.33–2.93
pilB2 (cpe2286) 1.143 2.950 5.233 0.74–1.44 1.07–3.62 2.98–5.68
pilC2 (cpe2285) 0.807 3.063 4.178* 0.79–2.29 1.95–3.41 1.52–3.02
pilT (cpe1767) 0.614* 1.735 0.942 0.77–1.28 1.27–2.09 0.87–1.64
cpe2277 0.679* 3.128 1.699* 0.72–1.87 1.82–3.18 0.88–1.61
lon (cpe2635) 0.795 0.939 1.130

*indicate a value out of fold change range but shows similar trends