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. Author manuscript; available in PMC: 2020 Jan 14.
Published in final edited form as: Circ Res. 2017 Mar 15;120(9):1414–1425. doi: 10.1161/CIRCRESAHA.116.310477

Figure 5. Vegfr3 deletion increases VEGFR2 levels.

Figure 5.

A, Western blotting of VEGFR2, VEGFR3 and heat shock protein 70 in the lungs of Vegfr3iΔ and control mice. B, Validation of vascular endothelial growth factor receptor (VEGFR) 3 silencing in human umbilical vein endothelial cells (HUVECs) after transduction with lentivirus expressing shFLT4 or scrambled control, using 2 different primary antibodies against VEGFR3. C–F, Quantification of VEGFR3 and VEGFR2 protein (C and D) and mRNA levels (E and F) in HUVECs transduced with lentivirus expressing shFLT4 or scrambled control. G, mRNA levels of NOTCH1 target genes HEY1, HES1 and DLL4 in VEGFR3-silenced HUVECs (4 independent experiments). H, Western blotting of VEGFR2 and pVEGFR2 from transduced HUVECs, stimulated with VEGF165 for 10 min before lysis. *P<0.05, **P<0.01, ***P<0.001, Error bars, SEM. Numbers (N) are indicated on the bars.