. 2020 Jan 1;11(4):906–918. doi: 10.7150/jca.33766

Table 1.

Correlation analysis of immunohistochemical expression of p53, p21, nm23 and VEGF with clinicopathological data.

		p53				p21				nm23				VEGF
Variables	Number	Negative	Positive	χ²	p	Negative	Positive	χ²	p	Negative	Positive	χ²	p	Negative	Positive	χ²	p
Gender
Male	377	149	228	0.230	0.631	274	103	0.670	0.413	40	337	0.493	0.482	85	292	2.437	0.119
Female	42	15	27			33	9			3	39			14	28
Age
≤46	229	83	146	1.778	0.182	157	72	5.722	0.017	20	209	1.282	0.258	57	172	0.447	0.504
>46	190	81	109			150	40			23	167			42	148
Race
Han	261	113	148	5.014	0.025	189	72	0.259	0.611	32	229	3.000	0.083	62	199	0.006	0.937
Minority	158	51	107			118	40			11	147			37	121
Smoking status
No	270	109	161	0.482	0.488	195	75	0.425	0.514	29	241	0.189	0.664	72	198	3.886	0.049
Yes	149	55	94			112	37			14	135			27	122
Drinking status
No	250	101	149	0.413	0.521	180	70	0.510	0.475	27	223	0.194	0.659	60	190	0.048	0.827
Yes	169	63	106			127	42			16	153			39	130
BMI
≤25	348	135	213	0.104	0.747	249	99	3.095	0.079	38	310	0.963	0.327	78	270	1.677	0.195
>25	71	29	42			58	13			5	66			21	50
AFP(ng/ml)^a
≤400	214	92	122	2.302	0.129	164	50	2.007	0.157	23	191	0.275	0.600	55	159	0.841	0.359
>400	175	62	113			123	52			16	159			38	137
Child pugh^b
A	337	127	209	1.503	0.220	248	88	0.425	0.514	31	305	1.371	0.242	77	259	0.848	0.357
B	56	26	30			39	17			8	48			16	40
BCLC stage
A	239	93	145	0.136	0.934	174	64	0.693	0.707	30	208	3.464	0.177	55	183	1.170	0.557
B	69	28	41			53	16			4	65			14	55
C	111	42	69			79	32			9	102			30	81
No. of tumors
Single (n=1)	308	119	189	0.124	0.725	227	81	0.111	0.739	36	272	2.566	0.109	76	232	0.707	0.400
Multiple (n>1)	111	45	66			80	31			7	104			23	88
Tumor size
≤5cm	138	58	80	0.721	0.396	110	28	4.358	0.037	17	121	0.945	0.331	31	107	0.154	0.694
>5cm	281	106	175			197	84			26	255			68	213
Cirrhosis
No	50	15	34	1.640	0.200	27	22	9.576	0.002	7	42	0.962	0.327	9	40	0.868	0.351
Yes	369	148	221			280	89			36	333			90	279
Pathological grade^c
Well	24	14	10	3.787	0.052	22	2	4.306	0.038	3	21	0.196	0.658	7	17	0.437	0.509
Moderately/Poorly	340	130	210			246	94			33	307			79	261
PVTT
No	352	141	211	1.500	0.827	258	94	0.070	0.999	37	315	2.087	0.720	79	273	5.325	0.256
Yes	67	3	8			8	3			1	10			1	10
Radical resection^d
No	169	89	149	0.491	0.484	172	66	0.420	0.517	24	214	0.034	0.853	63	175	2.644	0.104
Yes	238	69	100			127	42			18	151			33	136
Antiviral therapy
No	276	103	173	1.127	0.288	187	89	12.564	<0.001	36	240	6.791	0.009	65	211	0.003	0.959
Yes	143	61	82			120	23			7	136			34	109

^a information regarding AFP level was unavailable for 30 patients; ^b information regarding child-pugh was unavailable for 26 patients; ^c information regarding pathological grade was unavailable for 55 patients; ^d: information regarding radical resection was unavailable for 12 patients. HBV, hepatitis B virus; HCC, hepatocellular carcinoma; BMI, body mass index; AFP, α-fetoprotein; BCLC, Barcelona Clinic Liver Cancer; PVTT, portal vein tumor thrombus.