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. 2020 Jan 1;11(4):795–803. doi: 10.7150/jca.36905

Figure 2.

Figure 2

RNAi-induced RACK1 knockdown in melanoma cells. (A-B) RACK1 mRNA levels inhibited by siRNA in A375 and A875 cells was determined by RT-qPCR; (C-D) RACK1 protein levels inhibited by siRNA in A375 and A875 cells was determined by Western blot. *P<0.05, indicated a significant difference from the value of si-NC or untreated cells; (E-F). Knockdown efficiency of RACK1 protein in A375 and A875 cells was determined by fluorescence microscopy, red staining indicated RACK1 expression, cell nucleus was stained with Hoechst33258, the scale bar indicates 50 μm.