Figure 6.

Bazedoxifene combined with paclitaxel further suppressed glioblastoma tumor growth in orthotopic GBM mouse model. (A). Luciferase U251MG cells based orthotopic GBM mouse model showed that combination of bazedoxifene (BZA, 5 mg/kg) with paclitaxel (PTX, 10 mg/kg) remarkably suppressed tumor growth. (a). Representative IVIS images. (b). Tumor growth rate measured by IVIS. (c). HE staining. (d). Western blotting detection of EMT related markers and YAP signaling. (e). immunofluorescence staining of cleaved caspase 3. (B). Luciferase D456 cells based orthotopic GBM mouse model further confirmed that combination of bazedoxifene with paclitaxel remarkably suppressed tumor growth. (a). Representative IVIS images. (b). Tumor growth rate measured by IVIS. (c). HE staining. (d). Western blotting detection of EMT related markers and YAP signaling. (e). immunofluorescence staining of cleaved caspase 3. (C). Luciferase OMRP cells based orthotopic GBM mouse model further confirmed that combination of bazedoxifene with paclitaxel remarkably suppressed tumor growth. (a). Representative IVIS images. (b). Tumor growth rate measured by IVIS. (c). HE staining. (d). immunofluorescence staining of cleaved caspase 3. *p<0.1; **p<0.01; ***p<0.001