Figure 4.

Up-regulation of miR-1228 reverses the anti-cell proliferation effect on circ_100395 overexpressed (OE- miR-216a-5p) ovarian cancer cells. (A) The relative luciferase activity in 293 T cells after co-transfection with pmirGLO- circ_100395-WT or pmirGLO- circ_100395-MUT, along with miR-1228 mimics or miR-1228 NC. (B) The overexpression efficiency of miR-1228, after transfected with miR-1228 mimics or relative negative control (miR-1228 NC) by Lipo3000 in circ_100395-overexpressed ES-2 cell lines. (C) The up-regulated miR-1228 significantly inhibited the proliferation of circ_100395-overexpressed ES-2 cells in vitro using CCK-8 assay. (D & E) The overexpression of miR-1228 significantly increased the number of migrated and invaded cells in circ_100395-overexpressed ES-2 cells, measured by Transwell assay. (F) After the overexpression of miR-1228, the expression of the epithelial marker, E-cadherin was significantly reduced, while the mesenchymal markers, N-cadherin and Snail were dramatically increased in circ_100395-overexpressed ES-2 cell lines. *** P < 0.001