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. 2020 Jan 1;11(3):570–582. doi: 10.7150/jca.35631

Figure 2.

Figure 2

Activation of IL-6/STAT3 pathway played an important role in hypoxia-induced HIF-1α up-regulation. A. HIF-1α expression was verified to be down-regulated in SMMC7721 STAT3 siRNA cells compared to SMMC7721 control siRNA cells at the levels of mRNA and protein by both qRT-PCR and Western immunoblotting. B. mRNA expression microarray assay revealed that knockdown of STAT3 in SMMC7721 cells leaded to the down-regulation of HIF-1α signaling. C. Both qRT-PCR and Western immunoblotting assays displayed that IL-6 treatment increased expression of HIF-1α in Huh7 cells with up-regulation of p-STAT3 significantly, and after silencing STAT3, IL-6 did not reinforce HIF-1α expression in Huh7 cells any more. D. Both knockdown of STAT3 and the treatment of p-STAT3 inhibitor Stattic resulted in down-regulation of HIF-1α expression in MHCC97h cells.