Figure 6.

Knockdown of HIF-1α sensitized the anti-HCC effect of DOX in vitro and in vivo. A. MTT assay showed that HCC cells were repressed in a dose-dependent manner. B. MTT assay revealed that DOX administration (0.75 μM) leaded to a significant drop in cell viability in Huh7 cells, which was suppressed by HIF-1α up-regulation. C. The cytotoxicity of DOX on MHCC97h cells was also enhanced by siRNA-induced depletion of endogenous HIF-1α. D. HCC xenografts driven from Huh7 HIF-1α cells (PBS group) were dramatically larger than those from Huh7 Vector cells (PBS group). After DOX administration, the size of HCC xenografts from Huh7 HIF-1α cells were larger than those from Huh7 Vector cells.