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. 2020 Jan 1;11(5):1094–1103. doi: 10.7150/jca.30682

Figure 1.

Figure 1

Male mice are more susceptible to develop HCC than female mice. C57BL/6 mice were injected three times with DEN (100 mg/kg i.p.) starting at the age of 2 weeks, followed by six injections of CCl4 (0.5 ml/kg i.p.); mice were sacrificed different time points after DEN treatment (×200). B&C. Male and female mice were sacrificed 42 weeks after DEN injection. The appearance of liver tissue (A) and H&E staining (B) from DEN-induced mice were shown. (C) Tumor incidence, tumor number, and largest tumor size were assessed 42-weeks after DEN injection in female and male mice. (D) The proliferation marker Ki67, PCNA, and clinical indicators of HCC, AFP and Acta2, were analyzed by q-PCR. Data are represented as mean ± SEM (n=5). (E) Proliferation of hepatocytes indicated by Ki67 protein expression was analyzed by IHC (×400). * P < 0.05: Male-DEN/CCL4 versus Female-DEN/CCL4.