Figure 2.

The expression of both TLR4 and AR is increased in a male HCC model. (A) TLR4 and AR expression were evaluated by qPCR in untreated-female and male mice. (B) The mRNA of TLR4 and TLR4-positive hepatocytes from female and male mice treated with DEN were evaluated by qPCR and FACS. (C) AR levels were measured in DEN treated female and male mice by qRT-PCR. (D) NF-κB activation in hepatocytes from female and male mice was detected by Western Blot. Data are represented as mean ± SEM, (n=5). Bar graph shows quantification results.