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. 2020 Jan 1;11(5):1094–1103. doi: 10.7150/jca.30682

Figure 3.

Figure 3

TLR4 and AR up-regulate each other. (A) AR expression was analyzed by immunofluorescent staining in HepG2 and HepG2.2.15 cells; nuclear stain indicated by DAPI stain. The MERGE is the merge of DyLight 549 and DAPI (×400). (B) Western blot analysis showing the protein expression of TLR4 in HepG2 and HepG2.2.15 cells treated by different concentrations of DHT or E2 for 48 h. (C) TLR4 assay by FACS for HepG2 cells was performed following treatment with control or 100 nM DHT for 24h. Bar graph shows quantification results; data are represented as mean ± SEM. (D) Western blot analysis showing the protein expression of TLR4 and AR in HepG2 cells transfected with siRNA targeting AR (siAR), or scramble siRNA (Ctrl), for 48h. AR levels were measured in LPS treated HepG2 cells by qRT-PCR (E) and western blot (F). (G) TLR4 and AR protein expression levels were assayed by western blot in HepG2 cells treated with the TLR4 inhibitor TAK242. (H) Ki67 protein expression was analyzed by IHC at 23 weeks after DEN treatment in wild type (WT) and TLR4-/- male and female mice (×400).