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. 2020 Jan 1;11(5):1094–1103. doi: 10.7150/jca.30682

Figure 5.

Figure 5

DHT has no effect on the growth of Hepa1-6 cells. (A) AR expression was analyzed by immunofluorescent staining in Hepa1-6 cells; nuclear staining is indicated by DAPI stain. The MERGE is the merge of DyLight 549 and DAPI (×400). (B) Hepa1-6 cells were treated with LPS (10 µg/ml), DHT (100 nM), or with a combination for 2, 4, and 5 days, and the proliferation rate was assessed by the MTT assay. (C) Hepa1-6 cells were incubated with LPS (10 µg/ml) and DHT (100 nM) for 48 h, then the cell cycle distribution was analyzed by FACS. (D) Hepa1-6 cells treated with DHT, LPS, or with the combination indicated for 24 h, then Annexin V/PI staining was employed to detect apoptosis of the cells. (E) Hepa1-6 cells were treated with LPS (10 µg/ml), DHT (100 nM), or with a combination for 6 h. After 14 days, colony formation was assessed by Crystal Violet stain (×200). (F) Hepa1-6 cells were treated with LPS (10 µg/ml) and DHT (100 nM) for 6 h. The migration rate was evaluated by Crystal Violet staining (the scale bar is 100nm). Bar graph shows quantification results. Data are represented as mean ± SEM.