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. 2020 Jan 1;11(5):1027–1037. doi: 10.7150/jca.37401

Figure 1.

Figure 1

PRPS2 expression in prostate cancer tissues and prostate cancer cell lines. (A) Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), and with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, and in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 × 1 = 1. (B) Western blotting and real-time qPCR analysis of PRPS2 protein and mRNA expression in prostate cancer cell lines and normal prostate epithelial cell lines (RWPE-1). (*P < 0.05; **P < 0.01; ***P < 0.001). PRPS2: Phosphoribosyl pyrophosphate synthetase 2; TMA: tissue microarray.