Figure 3.

Knockdown of PRPS2 induced prostate cancer cell apoptosis and cell cycle arrest. (A) PRPS2 knockdown induced PCa cell cycle arrested in G₀/G₁ phase. (B) Representative micrographs (left) and quantification (right) of EdU incorporation assay. ^**P < 0.01, ^***P < 0.001. (C) PRPS2 knockdown increased apoptosis rate. ^*P < 0.05. (D) Western blot showed the protein levels of cell apoptosis-related proteins (cleavage of caspase-3, caspase-9, PARP, Bcl-2, Bax and p53) and cell cycle-related proteins (p27, CDK4 and cyclin D1) after down-regulated expression of PRPS2 in PC-3 (left) and DU145(right) cells. β-actin was served as loading control. NC, negative control.