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. 2020 Jan 1;11(5):1277–1287. doi: 10.7150/jca.35444

Figure 2.

Figure 2

M2 macrophages increase the HCC cells invasion and migration via altering the MMP9 expression. (A) Western bolt was used to check related genes expression in SK-HEP-1 cells after co-cultured with M2 macrophages. (B) Western blot was used to check MMP9 expression in HA22T cells after co-cultured with M2 macrophages. (C) Western blot was used to check MMP9 expression in SK-HEP-1 transfected with MMP9-shRNA#1 in co-culture system. (D-E) Invasion and migration capacity were measured in SK-HEP-1 transfected with MMP9 sh-RNA#1 in co-culture system. (F) Western blot was used to check MMP9 expression in SK-HEP-1 transfected with MMP9-shRNA#2 in co-culture system. (G-H) Invasion and migration capacity were measured in SK-HEP-1 transfected with MMP9 sh-RNA#2 in co-culture system. (I) Western blot was used to check MMP9 expression in HA22T overexpressed MMP9 in co-culture system. (J-K) Invasion and migration capacity were measured in HA22T overexpressed MMP9 in co-culture system. All quantifications are mean ± SD, *p < 0.05, **p < 0.01, ns: no significant difference.