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. 2020 Jan 1;11(5):1277–1287. doi: 10.7150/jca.35444

Figure 3.

Figure 3

M2 macrophages increase MMP9 expression of HCC cells via altering the miR-149-5p. (A) qRT-PCR was used to check MMP9 expression in SK-HEP-1 and HA22T cells after co-cultured with M2 macrophages. (B) qRT-PCR was used to check relative miRNAs expression in SK-HEP-1 cells after co-cultured with M2 macrophages. (C) Western blot was used to check MMP9 expression after over-expression miR-149-5p/483-3p/6734-3p in SK-HEP-1 cells in co-culture system. (D) Western blot was used to check MMP9 expression after over-expression miR-149-5p in HA22T cells in co-culture system. (E-F) Invasion and migration capacity were measured after over-expression miR-149-5p in SK-HEP-1 cells in co-culture system. (G) Western blot was used to check MMP9 expression after adding miR-149-5p inhibitor in HA22T cells in co-culture system. (H-I) Invasion and migration capacity were measured after adding miR-149-5p inhibitor in HA22T cells in co-culture system. All quantifications are mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 ns: no significant difference.