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. 2019 Feb 28;104(12):2493–2500. doi: 10.3324/haematol.2018.206250

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Figure 3. — Type 2B von Willebrand disease (vWF) induces desialylation of the integrin αIIbβ3. (A) Flow-cytometric analysis of wild-type (WT) platelets after being stained for GPIbα, GPVI, αIIbβ3, RCA and ECL with or without (w/o) OSGE treatment. Graphs are representative of three independent experiments. (B) RCA pull-down and western blot. Washed WT mouse platelets were treated with WT or 2B mouse vWF during 1 hour and then lyzed and prepared to RCA pull-down. Samples were subjected to western blot to analyze: (B) GPIbα and GPVI, (C) αIIb and β3. A histogram of αIIb and β3 in the pull-down expressed in desialylated form (from pull-down)/total form (from lysate). The mean±Standard Deviation values (n=3 experiments) were compared using a one-way ANOVA and Dunnett’s post-test: *P<0.05; ***P<0.001.