Figure 5.

A threshold of platelet desialylation is required to affect the platelet count. (A) Relative whole-blood platelet counts (dashed red line) and platelet RCA mean fluorescence intensity (MFI) (solid black line) in WT mice 1 hour after in vivo treatment with neuraminidase (n=3 mice for each concentration, values are quoted as the mean±Standard Deviation). The increase in RCA binding was calculated for each mouse as the ratio between the RCA MFI after treatment and the RCA MFI before treatment. (B) The panel focuses on the stability of the relative platelet count and the increase in RCA binding after treatment with low doses of neuraminidase (0, 2.5 and 5 mU/g of body weight). (C) The correlation between the relative platelet count and RCA binding after neuraminidase treatment (r²=0.95 in a linear regression).