Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 14;10:218. doi: 10.1038/s41598-019-57076-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ETV5 is required for cell migration and colony formation of neuroblastoma cells in vitro. (a) Wound confluence at 48 h after wound making on IncuCyte® of four different neuroblastoma cell lines after ETV5 knockdown (siETV5_63 and siETV5_65) compared to control vector (siCtrl). (n_{CLB-GA, NB-1, SH-SY5Y} = 3; n_SK-N-AS = 5; mean with error bars representing SD after error propagation). (b) Western blot analysis for ETV5 at 48 h after ETV5 knockdown (siETV5_63 and siETV5_65) in four different neuroblastoma cell lines. (vertical line indicates cropped image, full-length images are presented in Supplementary Fig. S7b). (c) Phase contrast imaging of the scratch wound on IncuCyte® for CLB-GA at start (0 h) and after 48 h of ETV5 knockdown (siETV5_63 and siETV5_65) compared to control vector (siCtrl). Blue represents the initial scratch wound, yellow represents the scratch wound masking. (d,e) Colony formation analysis of two different NB cell lines after ETV5 knockdown with shETV5 compared to control vector (shCtrl SHC002; left panel) and after ETV5 knockdown with siETV5_63 and siETV5_65 compared to control vector (siCtrl; middle and right panel). The upper panel shows the cristal violet staining, the lower panel shows the relative colony counts. (n_shRNA = 2; n_{siRNA, SK-N-AS} = 4; n_{siRNA, SH-SY5Y} = 3; mean with error bars representing SD after error propagation) (*p < 0.05; **p < 0.01; ***p < 0.001).