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. 2020 Jan 14;10:275. doi: 10.1038/s41598-019-57145-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profile during 24 hours of (a) PhGI1 and PhGI2 in wild type plants and (b) PhGI1, (c) PhGI2, (d) PhCHL, (e) PhLHY, (f) PhTOC and (g) PhELF4 in iRNA::PhGI1 T1 lines 3.7, 4.7, 8.1 compared to expression in the wild type (from ZT 0 to ZT 15 of light and from ZT 15 to ZT 24 of dark). Expression represents the normalized expression NE according to the formula (NE) = 2^-(Ct experimental – Ct normalization). Three samples were analyzed for each time point and error bars indicate the standard deviation. Asterisks indicate statistical significance between wild type and iRNA lines with *P < 0.05; **P < 0.01; ***P < 0.001 according to Student’s T-test.