Table 2.
Comparison of vegetative parameters between wild type and the silenced PhGI1 in T1 and T2 generation.
| Genotype: | W.T. | iRNA::PhGI1 | % GI1 versus W.T. | P value | |
|---|---|---|---|---|---|
| Plant Height (cm) | T1 | 40.9 ± 0.8 | 44 ± 4.4 | +7.6 | 2,89E-02 |
| T2 | 44.7 ± 6.2 | 44.6 ± 4.9 | −0.2 | 9,93E-01 | |
| Basal Internode (mm) | T1 | 12.61 ± 0.91 | 16.93 ± 0.51 | +34.4 | 2,46E-20 |
| T2 | 14.28 ± 1.97 | 17.16 ± 0.79 | +20.3 | 1,40E-03 | |
| Median Internode (mm) | T1 | 16.30 ± 0.56 | 10.17 ± 0.40 | −37.6 | 3,57E-30 |
| T2 | 15.73 ± 1.3 | 12.44 ± 0.98 | −20.8 | 1,42E-05 | |
| Apical Internode (mm) | T1 | 20.5 ± 1.07 | 13.60 ± 0.31 | −33.7 | 2,51E-23 |
| T2 | 27.9 ± 1.63 | 23.81 ± 0.82 | − 14.6 | 2,77E-05 | |
| N° of leaves to the 1° flower | T1 | 37 ± 1.4 | 36.5 ± 1.2 | −1.4 | 7,62E-01 |
| T2 | 28.0 ± 1.0 | 28.7 ± 1.44 | +2.5 | 3,74E-01 | |
| N° of axillary meristems | T1 | 12.5 ± 1.3 | 27 ± 2.08 | +116 | 3,78E-06 |
| T2 | 15.3 ± 1.5 | 26.6 ± 3.48 | +73.9 | 1,89E-04 | |
| N° of branches | T1 | 2 ± 0.0 | 2.4 ± 0.57 | +20 | 2,73E-01 |
| T2 | 6.7 ± 0.55 | 6.6 ± 0.7 | −1.5 | 7,80E-01 | |
| Basal Leaves length (mm) | T1 | 64.57 ± 1.04 | 91.75 ± 5.24 | +42 | 4,42E-33 |
| T2 | 90.99 ± 1.55 | 112.07 ± 6.05 | +23.2 | 3,31E-30 | |
| Basal leaves width (mm) | T1 | 40.93 ± 0.72 | 50.96 ± 1.53 | +24.5 | 4,97E-30 |
| T2 | 49.92 ± 1.6 | 53.78 ± 1.19 | +7.7 | 4,03E-05 | |
| Median Leaves length (mm) | T1 | 72.72 ± 2.84 | 75.64 ± 1.66 | +4.0 | 8,23E-02 |
| T2 | 65.38 ± 1.74 | 70.25 ± 1.58 | +7.4 | 2,08E-07 | |
| Median leaves width (mm) | T1 | 43.82 ± 1.68 | 48.05 ± 1.39 | +9.7 | 1,13E-08 |
| T2 | 36.89 ± 1.45 | 39.84 ± 1.22 | +8.0 | 1,97E-05 | |
| Apical leaves length (mm) | T1 | 38.77 ± 1.31 | 48 ± 2.91 | +24 | 1,49E-22 |
| T2 | 33.15 ± 1.82 | 36.08 ± 0.81 | +8.8 | 1,36E-03 | |
| Apical leaves width (mm) | T1 | 22.54 ± 0.97 | 30.53 ± 2.54 | +35 | 2,02E-23 |
| T2 | 17.86 ± 0.59 | 21.96 ± 0.64 | +23 | 2,69E-11 | |
| Basal leaves Chlorophyll | T1 | 22.38 ± 1.09 | 14.89 ± 1.14 | −33.5 | 1,05E-23 |
| T2 | 17.11 ± 1.08 | 13.84 ± 0.77 | −19.1 | 7,11E-05 | |
| Median leaves Chlorophyll | T1 | 31.04 ± 2.29 | 37.26 ± 1.07 | +20 | 1,67E-25 |
| T2 | 20.14 ± | 24.99 ± 0.95 | +24.1 | 2,97E-05 | |
| Apical leaves Chlorophyll | T1 | 21.27 ± 0.95 | 38.19 ± 1.74 | +79.5 | 2,76E-45 |
| T2 | 34.47 ± 0.90 | 43.57 ± 1.03 | +26.4 | 1,96E-14 | |
Data are given as averages of at least three biological replicates of all silenced plants. The height was calculated from the base to the first flowering meristem. when the first flowering event occurred. The number of total axillary meristems was calculated between the base and the first apical flowering meristem. P values ≤ 0,05 according to Students T-test were considered as significant.