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. 2020 Jan 14;11:277. doi: 10.1038/s41467-019-14068-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic diagrams showing various FMN2 constructs used in this study. FH1: formin homology domain 1; FH2: formin homology domain 2; FSI: formin–spire interaction. b Images showing the localization of full length FMN2 (green), aa 1–135 region of FMN2 (cortical localization domain: CLD) and aa 275–734 (spindle periphery localization domain: SLD) in wild-type oocytes. Scale bar, 20 µm. c Representative images (green) show the localization of FMN2^∆CLD and FMN2^∆SLD in wild-type oocytes. Representative results, n = 23 (left) and 7 (right). Scale bar, 20 µm. d Representative images show the effect of SLD and CLD expressions on full-length FMN2 localization. Representative results, n = 5 (left) and 20 (right). Scale bar, 20 µm. e Representative time-lapse images of spindle/chromosomes migration in wild-type oocytes or oocytes with SLD or CLD expression. Scale bar, 20 µm. f Quantification of the percentage of oocytes that underwent spindle migration after SLD and CLD expressions in wild-type oocytes from four independent experiments. g Chromosomes were tracked in oocytes as shown in e and the chromosomes movements were plotted. Data are represented as mean ± SD, from three independent experiments. h The speed of spindle/chromosomes migration was analyzed from the plots in g. Data are from three independent experiments. i Representative time-lapse images of chromosomes migration after FMN2^∆SLD and FMN2^∆CLD expression in Fmn2^−/− oocytes. Representative results, n = 16 (upper) and 17 (lower). Scale bar, 20 µm. j The centroids of chromosomes were tracked and chromosomes movements were plotted as the distance from the initial central position over time. Data are from three independent experiments. k The speed of chromosomes migration was analyzed from the plots in j. l Quantification of the percentage of oocytes that underwent spindle migration after FMN2^∆SLD, FMN2^∆CLD, FMN2^FL expression in Fmn2^−/− oocytes. Data are from at least three independent experiments. All the data in this figure were analyzed by one side ANOVA, Tukey’s multiple comparisons test. Data are represented as mean ± SD, and oocyte numbers are indicated in brackets. Source data are provided as a Source Data file.