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. 2020 Jan 14;11:267. doi: 10.1038/s41467-019-14135-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Illustration and representative laser scanning confocal microscope (LSCM) images of dual-labeled AP binding to the S. Enteritidis. b Electrophoretic analysis of the feasibility of APC-Cas for S. Enteritidis detection. M: DNA marker, L1: AP; L2: AP + primer; L3: AP + primer + KF DNA polymerase; L4: AP + primer + KF DNA polymerase + S. Enteritidis; L5: AP + primer + KF DNA polymerase + S. Enteritidis + T7 RNA polymerase. ‘ + ’ means presence, ‘-’ means absence. c Fluorescence measurement of LbuCas13a activity. RNase A was used as positive control for the degradation of RNA reporter probe. d Comparison of seven APs with varied stem-length. Data represent mean ± s.d., n = 3, three technical replicates.