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. 2020 Jan 14;11:267. doi: 10.1038/s41467-019-14135-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The fluorescence signal of APC-Cas for detection of S. Enteritidis at single-level. b Fluorescence growth rate (V_g) of APC-Cas for single S. Enteritidis detection. c Real-time kinetic measurement of APC-Cas. Different amount of S. Enteritidis (0–10⁵ CFU) was introduced into the system at t = 0 min. d Linear analysis of S. Enteritidis detection by APC-Cas. e Real-time PCR initiated by amount of S. Enteritidis from 40 to 4 × 10⁷ CFU. f Linear analysis of S. Enteritidis detection by real-time PCR. Data represent mean ± s.d., n = 3, three technical replicates.