. 2020 Jan 14;10:231. doi: 10.1038/s41598-019-57112-4

Table 2.

Best combination of housekeeping miRNAs for each group of samples yielded by software analyses.

Groups	Best pair of housekeeping genes by software						Best pair of housekeeping gene by visual inspection	Best trio of housekeeping genes by visual inspection
Groups	NormFinder	GeNorm	RefFinder	ΔCt method	Bestkeeper	DataAssist	Best pair of housekeeping gene by visual inspection	Best trio of housekeeping genes by visual inspection
All	miR-25 + miR-26a	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-26a	miR-26a + miR-191	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-25 + miR-26a or miR-20a + miR-26a + miR-191
CY	miR-20a + miR-26a	miR-20a + miR-25	miR-20a + miR-25	miR-20a + miR-25	miR-25 + miR-191	miR-20a + miR-25	miR-20a + miR-25	miR-20a + miR-25 + miR-26a or miR-20a + miR-26a + miR-191
NC	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-191	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-26a + miR-191
HT	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-191	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-26a + miR-191
WT	miR-20a + miR-26a	miR-20a + miR-25	miR-25 + miR-26a	miR-25 + miR-26a	miR-20a + miR-25	miR-20a + miR-25	miR-20a + miR-25	miR-20a + miR-25 + miR-26a
SC	miR-25 + miR-191	miR-25 + miR-191	miR-25 + miR-191	miR-25 + miR-191	miR-25 + miR-191	miR-25 + miR-191	miR-25 + miR-191	—
CO	miR-20a + U6	miR-26a + miR-191	miR-20a + miR-26a	miR-20a + miR-26a	U6 + miR-25	miR-20a + miR-26a	miR-20a + miR-26a	miR-20a + miR-25 + miR-26a or miR-20a + miR-26a + miR-191 or miR-20a + miR-26a + U6

All, all samples; CY, cystic; NC, non-cystic; HT, haploinsuficient; WT, wild-type; SC, severely cystic phenotype; CO, severely cystic phenotype controls.