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. 2020 Jan 14;10:261. doi: 10.1038/s41598-019-57136-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The spatial distribution of threonine-O-3-phosphate as agglomerates in a 3D reconstruction of a nanoparticulate cluster. The reconstruction shows the whole NP agglomerate and successive phosphate agglomerate distribution (depicted in green) in and around deeper layers of the spherical Au-NP. (A) Slicing the spherical NPs with different z-micro stacks and 3D reconstruction from top to bottom as shown in top view: Au particle reconstructed from Au⁺ signals (orange, solid); threonine-O-3-phosphate aureate (green). (B) Zoomed-in view spatially isolated threonine-O-3-phosphate aureate showing m/z 417.06 green signal within a single Au-NP cluster.