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. 2020 Jan 14;10:261. doi: 10.1038/s41598-019-57136-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The constructed ion image reveals quasi spherical Au-NPs with increasing Au ions to 1 mM Au ions. (A,B) The topography is displayed by total ion reconstruction image of the A549 cell surface. Inset show SEM image of quasi-spherical nanostructure. (C) 3D reconstructed ion image of a single cluster with the Au⁺ signals (m/z 196.97) picked from biomineralized cell surface. (D) The zoomed in-depth 3D reconstruction overlay of cluster shown in (C) in side view of spatiotemporal Au⁺ signals (orange) with threonine-O-3-phosphate aureate (green).