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. 2020 Jan 14;10:261. doi: 10.1038/s41598-019-57136-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SEM and ToF-SIMS analysis of de novo biomineralized nanostructures at 2 mM Au ions: delta and rhombus shaped particles. (A–D) SEM and EDX chemical mapping to confirm anisotropic rhombus/delta shape Au nanoplate formation (Scale bar 1 micron). (E,F) The SIMS signals from Au⁺ (E) and threonine-O-3-phosphate aureate (F). (G) Reconstructed ToF-SIMS ion image from the A549 cell surface demonstrating spherical Au particles. (H) The 3D reconstruction and overlay of Au⁺ signal (orange) and threonine-O-3-phosphate aureate (green) from red square ROI from (G).