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. 2020 Jan 7;7:372. doi: 10.3389/fbioe.2019.00372

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Copyright © 2020 Castaño-Cerezo, Fournié, Urban, Faulon and Truan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dose-response curves obtained with the strongest benzoic acid derivative effectors of sBAD expressed in S. cerevisiae. mCitrine fluorescence was measured by flow cytometry. The relative fluorescence of mCitrine was measured with cells grown at different concentration of (A) pHBA, (B) pABA, (C) Benzoic acid, and (D) Aspirin is compared with the control condition. In (E), the affinity and dynamic parameters calculated from different the curves dose response are presented. Values are calculated as the mean of three biological replicates.