Figure 5.

Effect of DGAT inhibition on oleic acid oxidation. Human myotubes were grown and differentiated on 12- or 24-well tissue culture plates. At day 7 of differentiation, myotubes were incubated with 100 µM [¹⁴C]oleic acid (0.5 µCi/ml, 100 µM) for 4 h in presence or absence of D1i (1 µM) or D2i (10 µM), respectively. Complete oxidation (CO₂ production) and β-oxidation (ASMs) were measured. (A–C) Complete oxidation (CO₂) of oleic acid. (D–F) ASMs, which reflects incomplete oxidation (β-oxidation), of oleic acid. Results are presented as mean ± SEM from n = 5–6 (D1i) and n = 8 (D2i) individual experiments with 8 separate wells for each condition, as absolute values (nmol/mg protein) (A,B and D,E) and normalized to control (C,F). *p < 0.05 vs control, paired t-test. ASMs, acid soluble metabolites; D1i, DGAT1 inhibitor; D2i, DGAT2 inhibitor.