Figure 6.

Effect of DGAT inhibitors on glucose metabolism. (A–B) Human myotubes were grown on 96-well tissue culture plates. At day 7, myotubes were incubated with [¹⁴C(U)]glucose (0.5 µCi/ml, 200 µM) and D1i or D2i for 4 h. Oxidation, measured as CO₂ production from [¹⁴C(U)]glucose (A) and cell-associated radioactivity from [¹⁴C(U)]glucose (B) was measured after 4 h treatment with DGAT inhibitors; D1i (1 µM) and D2i (10 µM). (C) Alternatively, cells were grown in 12-well tissue culture plates. At day 7, myotubes were starved for 90 min (DMEM without glucose) before incubation for 3 h with D-[¹⁴C(U)]glucose (1 µCi/ml, 5.5 mM) in presence or absence of D1i (1 µM) or D2i (10 µM) and with or without insulin (100 nM). Results are presented as mean ± SEM from n = 9 (A,B) individual experiments with 8 separate wells for each condition, or n = 4 (C) individual experiments with duplicate wells for each condition. Absolute values for glycogen synthesis: control 11.0 ± 4.5 and with insulin 22.4 ± 10.8 nmol/mg cell protein. *p < 0.05 vs control, paired t-test.