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. 2020 Jan 14;10:207. doi: 10.1038/s41598-019-57052-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Constitutive expression of oncogenic KRAS under the control of the EF1-tTA in the mammary gland and other MMTV-Cre target tissues. (A) Brightfield and GFP fluorescent images (upper, middle panels) of an unfixed #4 inguinal mammary gland of MMTV-Cre EF1-LSL-tTA TetO-KRAS^G12D TetO-H2B-GFP quadruple transgenic female; Carmine alum-stained wholemount of the same tissue (lower panel); LN, lymph node; bars, 1 mm. (B) Brightfield (upper), GFP (middle), and overlay (bottom) images of a quadruple transgenic mouse that developed multiple skin tumors.